Scaling Innovation Leveraging Bacto™ CD Supreme Supplementation and Bacto™ CD Feed to Unlock pDNA

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Consistent upstream processing is critical for scalable pDNA manufacturing. This presentation video shows how Afrigen Biologics optimized pDNA production through media selection, supplementation, and fed-batch cultivation.

You’ll learn how to:

• Compare LB and chemically defined media for pDNA production
• Optimize cultivation parameters and fed-batch strategies
• Improve supercoiled plasmid quality during scale-up
• Build a reproducible process for GMP-ready manufacturing

Discover how these process improvements established a scalable upstream platform for GMP-ready mRNA manufacturing and future technology transfer